Código HTML:
function Suma2(isChecked, myValue){
myValue=myValue.replace(",","");
var total=0;
tot = parseFloat(document.sumar2.total2.value);
myValue = parseFloat(myValue);
if (isChecked)
total = tot + myValue;
else
total = tot - myValue;
document.sumar2.total2.value = total.toFixed(2);
}